摘要
本实验通过对禽源不同血清型大肠杆菌对同一药物的敏感性检测的研究,探讨大肠杆菌不同血清型对多种药物敏感性,用来科学指导临床用药,治疗禽大肠杆菌病。实验共有25株菌种鉴定出9个菌株6血清型血清型,进行26种药物药敏试验,结果得出6个血清型对先锋Ⅴ、氯霉素、菌必治等10种药物均表现高敏,对另外10种药物均表现耐药性,还有6种药物敏感性表现不一致,根据试验结果治疗大肠杆菌病推荐使用高敏的10种药物及敏感性较高的氧氟沙星、卡那霉素。
关键词 大肠杆菌 血清型 药敏试验
Abstract
This paper studied the sensitivities of different serotypes of avian E. coli for the same drug by the drug sensitivity tests, and provide theoretical basis for clinical treatment. In this experiment, 6 serotypes of the bacteria were gained from nine strains of 25 species, and the high-sensitivity drugs were screened from 26 antibiotics for the six E. coli bacteria. The results showed that 6 serotypes of the bacteria were high-sensitivity for 10 kinds of drugs such as pioneer V, chloramphenicol and junbizhi, showed resistance to 10 kinds of drugs, and were inconsistent sensitivity for the other 6 kinds of drugs. From those results, 10 high-sensitivity drugs, ofloxacin and kanamycin were recommended to use in treating the E. coli disease.
Key words:E. coli; Serotype; Drug Sensitivity Test
目录
摘要 1
ABSTRACT 2
1 前 言 4
2 材料与方法 5
2.1 材料 5
2.2 方法 6
2.3 操作步骤 8
3 结果与分析 13
3.1 病料采集 13
3.2 初步镜检结果 14
3.3 菌种筛选结果与菌种纯化增殖培养结果 14
3.4 纯化菌种镜检结果和显色培养结果 15
3.5 生化鉴定结果 16
3.6 血清型鉴定结果 18
3.7 药敏试验结果 20
4 讨 论 23
参 考 文 献 24
1 前 言
大肠埃希氏菌习惯称为大肠杆菌[1],分类于肠杆菌科,归属于埃希氏菌属,为禽类肠道中的正常栖居菌,形态为两端钝圆的小杆菌,大小2 μm~3 μm[1],无芽孢,大多数菌株有动力,周身鞭毛,分为普通菌毛与性菌毛,有些菌株有多糖类包膜,革兰氏阴性菌,是一种条件性病原微生物,广泛存在于饲料、饮水、垫草、粪便和禽舍的灰尘中,也作为常在菌存在于正常动物的肠道中,当机体抵抗力下降,特别是应激情况下(如长途运输、气温骤变、饲喂不当等),可引起肠道外感染,某些血清型菌株的致病性强,引起腹泻,统称致病大肠杆菌。

您当前的位置:
